Part:BBa_K1021032:Design

PT7+nptII

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 848
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 681
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 530
  Illegal SapI.rc site found at 740
  

Design Notes

An RBS was excluded because this part was designed for T7-based expression in fungi.

Source

The promoter comes from the T7 virus; the gene, originating from E. coli K12, was taken from the pNG transformation vector.